Analysis of freezable water content by DSC for apple dormant bud cryopreservation
نویسندگان
چکیده
منابع مشابه
Freezing of water in dormant vegetative apple buds in relation to cryopreservation.
Various empirical prefreezing protocols have been used to facilitate cryopreservation of dormant buds from woody plants. The objective of this research was to determine the quantity of water remaining in liquid phase, under different prefreezing conditions using pulsed nuclear magnetic resonance spectroscopy of dormant apple (Malus domestica Mill.) buds from three cultivars. During prefreezing,...
متن کاملCryopreservation of winter-dormant apple buds: II - tissue water status after desiccation at -4C and before further cooling.
The established protocol for the cryopreservation of winter-dormant Malus buds requires that stem explants, containing a single, dormant bud are desiccated at -4 degree C, for up to 14 days, to reduce their water content to 25-30 percent of fresh weight. Using three apple cultivars, with known differences in response to cryopreservation, the pattern of evaporative water loss has been characteri...
متن کاملAnatomical Study of Primary Bud Necrosis in Vitis vinifera L. cv. Askari in Winter Dormant Bud
Primary bud necrosis in grapevine is a physiological disorder observed in the compound buds. This study was conducted to investigate the anatomical changes in cv. Askari grapevine in winter dormant buds. Bud samples were taken from Askari grapevine at Sisakht vineyards, south-western of Iran. Compound buds were dissected and assessed under a binocular microscope at 10-40x magnification and a di...
متن کاملCryopreservation of Vegetative Dormant Winter Buds
Cryopreservation of vegetative winter buds is an alternative method to preserving plant species in the field. In this method, nodal sections of scionwood are stored in liquid nitrogen vapor. Cryopreservation of the vegetative dormant winter buds involves low maintenance costs and virtually eliminates the risk of germplasm loss. At the NCGRP, this method is routinely used to preserve Malus sp. (...
متن کاملOptimization of Water Content for the Cryopreservation Of Allium sativum In Vitro Cultures by Encapsulation-Dehydration.
BACKGROUND There is a general requirement to determine and correlate water content to viability for the standardization of conservation protocols to facilitate effective cryostorage of plant germplasm. OBJECTIVE This study examined water content as a critical factor to optimize the cryostorage of Allium sativum. MATERIALS AND METHODS Stem discs were excised from post-harvest, stored bulbs p...
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ژورنال
عنوان ژورنال: Horticultural Science
سال: 2019
ISSN: 0862-867X,1805-9333
DOI: 10.17221/146/2018-hortsci